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phospho mtor  (Bioss)


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    Bioss phospho mtor
    The protein expression of <t>PI3K,</t> <t>AKT,</t> <t>mTOR</t> and their respective phosphorylated forms detected by Western blotting
    Phospho Mtor, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho mtor/product/Bioss
    Average 94 stars, based on 27 article reviews
    phospho mtor - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder"

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-026-06096-2

    The protein expression of PI3K, AKT, mTOR and their respective phosphorylated forms detected by Western blotting
    Figure Legend Snippet: The protein expression of PI3K, AKT, mTOR and their respective phosphorylated forms detected by Western blotting

    Techniques Used: Expressing, Western Blot

    Immunofluorescence analysis of GHR and SSTR2 in the prefrontal cortex. A Proposed signaling pathway of GH in regulating the PI3K/Akt/mTOR axis (KEGG Entry: map04935). B Quantitative analysis of the expression levels of GHR and SSTR2. C Representative immunofluorescence images of GHR and SSTR2 (50.0×, Scale bar: 20 μm). The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group
    Figure Legend Snippet: Immunofluorescence analysis of GHR and SSTR2 in the prefrontal cortex. A Proposed signaling pathway of GH in regulating the PI3K/Akt/mTOR axis (KEGG Entry: map04935). B Quantitative analysis of the expression levels of GHR and SSTR2. C Representative immunofluorescence images of GHR and SSTR2 (50.0×, Scale bar: 20 μm). The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

    Techniques Used: Immunofluorescence, Expressing, Control

    Immunofluorescence analysis of GH and p-AKT/GH and p-mTOR co-localization in the prefrontal cortex. A Representative immunofluorescence images of GH and p-Akt (50.0×, Scale bar: 20 μm). B Quantification of the Pearson’s correlation coefficient (R value) for GH and p-Akt co-localization. C Representative immunofluorescence images of GH and p-mTOR (50.0×, Scale bar: 20 μm). D Quantification of the Pearson’s correlation coefficient (R value) for GH and p-mTOR co-localization from. The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group
    Figure Legend Snippet: Immunofluorescence analysis of GH and p-AKT/GH and p-mTOR co-localization in the prefrontal cortex. A Representative immunofluorescence images of GH and p-Akt (50.0×, Scale bar: 20 μm). B Quantification of the Pearson’s correlation coefficient (R value) for GH and p-Akt co-localization. C Representative immunofluorescence images of GH and p-mTOR (50.0×, Scale bar: 20 μm). D Quantification of the Pearson’s correlation coefficient (R value) for GH and p-mTOR co-localization from. The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

    Techniques Used: Immunofluorescence, Control



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    Image Search Results


    The protein expression of PI3K, AKT, mTOR and their respective phosphorylated forms detected by Western blotting

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

    doi: 10.1007/s00018-026-06096-2

    Figure Lengend Snippet: The protein expression of PI3K, AKT, mTOR and their respective phosphorylated forms detected by Western blotting

    Article Snippet: For immunofluorescence staining, free-floating hypothalamic sections were co-incubated with primary antibodies against somatostatin (SST) (1:100, rabbit, bs-37040R, Bioss) and growth hormone-releasing hormone (GHRH) (1:100, rabbit, bs-0205R, Bioss), while prefrontal cortical sections were incubated with primary antibodies targeting growth hormone (GH) (1:1000, rabbit, GB113303-100, Servicebio), phospho-AKT (1:100, rabbit, bs-0876R, Bioss), phospho-mTOR (1:100, rabbit, bs-3495R, Bioss), growth hormone receptor (GHR) (1:100, rabbit, bs-0654R, Bioss), and somatostatin receptor 2 (SSTR2) (1:100, rabbit, bs-10986R, Bioss).

    Techniques: Expressing, Western Blot

    Immunofluorescence analysis of GHR and SSTR2 in the prefrontal cortex. A Proposed signaling pathway of GH in regulating the PI3K/Akt/mTOR axis (KEGG Entry: map04935). B Quantitative analysis of the expression levels of GHR and SSTR2. C Representative immunofluorescence images of GHR and SSTR2 (50.0×, Scale bar: 20 μm). The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

    doi: 10.1007/s00018-026-06096-2

    Figure Lengend Snippet: Immunofluorescence analysis of GHR and SSTR2 in the prefrontal cortex. A Proposed signaling pathway of GH in regulating the PI3K/Akt/mTOR axis (KEGG Entry: map04935). B Quantitative analysis of the expression levels of GHR and SSTR2. C Representative immunofluorescence images of GHR and SSTR2 (50.0×, Scale bar: 20 μm). The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

    Article Snippet: For immunofluorescence staining, free-floating hypothalamic sections were co-incubated with primary antibodies against somatostatin (SST) (1:100, rabbit, bs-37040R, Bioss) and growth hormone-releasing hormone (GHRH) (1:100, rabbit, bs-0205R, Bioss), while prefrontal cortical sections were incubated with primary antibodies targeting growth hormone (GH) (1:1000, rabbit, GB113303-100, Servicebio), phospho-AKT (1:100, rabbit, bs-0876R, Bioss), phospho-mTOR (1:100, rabbit, bs-3495R, Bioss), growth hormone receptor (GHR) (1:100, rabbit, bs-0654R, Bioss), and somatostatin receptor 2 (SSTR2) (1:100, rabbit, bs-10986R, Bioss).

    Techniques: Immunofluorescence, Expressing, Control

    Immunofluorescence analysis of GH and p-AKT/GH and p-mTOR co-localization in the prefrontal cortex. A Representative immunofluorescence images of GH and p-Akt (50.0×, Scale bar: 20 μm). B Quantification of the Pearson’s correlation coefficient (R value) for GH and p-Akt co-localization. C Representative immunofluorescence images of GH and p-mTOR (50.0×, Scale bar: 20 μm). D Quantification of the Pearson’s correlation coefficient (R value) for GH and p-mTOR co-localization from. The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

    doi: 10.1007/s00018-026-06096-2

    Figure Lengend Snippet: Immunofluorescence analysis of GH and p-AKT/GH and p-mTOR co-localization in the prefrontal cortex. A Representative immunofluorescence images of GH and p-Akt (50.0×, Scale bar: 20 μm). B Quantification of the Pearson’s correlation coefficient (R value) for GH and p-Akt co-localization. C Representative immunofluorescence images of GH and p-mTOR (50.0×, Scale bar: 20 μm). D Quantification of the Pearson’s correlation coefficient (R value) for GH and p-mTOR co-localization from. The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

    Article Snippet: For immunofluorescence staining, free-floating hypothalamic sections were co-incubated with primary antibodies against somatostatin (SST) (1:100, rabbit, bs-37040R, Bioss) and growth hormone-releasing hormone (GHRH) (1:100, rabbit, bs-0205R, Bioss), while prefrontal cortical sections were incubated with primary antibodies targeting growth hormone (GH) (1:1000, rabbit, GB113303-100, Servicebio), phospho-AKT (1:100, rabbit, bs-0876R, Bioss), phospho-mTOR (1:100, rabbit, bs-3495R, Bioss), growth hormone receptor (GHR) (1:100, rabbit, bs-0654R, Bioss), and somatostatin receptor 2 (SSTR2) (1:100, rabbit, bs-10986R, Bioss).

    Techniques: Immunofluorescence, Control

    A: western blot results showed that argon treatment significantly upregulated the levels of p-PI3K, p-Akt, and p-mTOR. B: Bar chart for A; C: western blot results of PI3K/Akt/mTOR pathway factors and autophagy factors in N 2, N 2 combined with SC79, and N 2 combined with MHY1485 group; D: Bar chart for C. E: western blot results of in argon, argon combined with MK2206 and argon combined with rapamycin groups. F: Bar chart for E. MHY: MHY1485; Rapa: rapamycin.

    Journal: bioRxiv

    Article Title: Argon inhibits autophagy and improves cerebral ischemia-reperfusion injury via PI3K/Akt/mTOR pathway

    doi: 10.1101/2025.10.31.685964

    Figure Lengend Snippet: A: western blot results showed that argon treatment significantly upregulated the levels of p-PI3K, p-Akt, and p-mTOR. B: Bar chart for A; C: western blot results of PI3K/Akt/mTOR pathway factors and autophagy factors in N 2, N 2 combined with SC79, and N 2 combined with MHY1485 group; D: Bar chart for C. E: western blot results of in argon, argon combined with MK2206 and argon combined with rapamycin groups. F: Bar chart for E. MHY: MHY1485; Rapa: rapamycin.

    Article Snippet: The first antibodies included: P62 (Abcam, 1:1000, rabbit derived polyclonal antibody, ab91526), LC3 (Sigma Aldrich, 1:500, rabbit derived polyclonal antibody, ABC929), β-actin (MCE, 1:5000, mouse monoclonal antibody, HY-P80438), PI3K (Abcam, 1:1000, rabbit derived monoclonal antibody, ab302958), p-PI3K (Abcam, 1:1000, rabbit derived polyclonal antibody, ab138364), Akt (MCE, 1:2000, rabbit monoclonal antibody), p-Akt (MCE, 1:1000, rabbit monoclonal antibody, HY-P80276), mTOR (MCE, 1:1000, rabbit polyclonal antibody, HY-P80276), p-mTOR (MCE, 1:1000, rabbit monoclonal antibody, HY-P80837).

    Techniques: Western Blot

    A: western blot results showed that argon treatment significantly upregulated the levels of p-PI3K, p-Akt, and p-mTOR. B: Bar chart for A; C: western blot results of PI3K/Akt/mTOR pathway factors and autophagy factors in N 2, N 2 combined with SC79, and N 2 combined with MHY1485 group; D: Bar chart for C. E: western blot results of in argon, argon combined with MK2206 and argon combined with rapamycin groups. F: Bar chart for E. MHY: MHY1485; Rapa: rapamycin.

    Journal: bioRxiv

    Article Title: Argon inhibits autophagy and improves cerebral ischemia-reperfusion injury via PI3K/Akt/mTOR pathway

    doi: 10.1101/2025.10.31.685964

    Figure Lengend Snippet: A: western blot results showed that argon treatment significantly upregulated the levels of p-PI3K, p-Akt, and p-mTOR. B: Bar chart for A; C: western blot results of PI3K/Akt/mTOR pathway factors and autophagy factors in N 2, N 2 combined with SC79, and N 2 combined with MHY1485 group; D: Bar chart for C. E: western blot results of in argon, argon combined with MK2206 and argon combined with rapamycin groups. F: Bar chart for E. MHY: MHY1485; Rapa: rapamycin.

    Article Snippet: The first antibodies included: P62 (Abcam, 1:1000, rabbit derived polyclonal antibody, ab91526), LC3 (Sigma Aldrich, 1:500, rabbit derived polyclonal antibody, ABC929), β-actin (MCE, 1:5000, mouse monoclonal antibody, HY-P80438), PI3K (Abcam, 1:1000, rabbit derived monoclonal antibody, ab302958), p-PI3K (Abcam, 1:1000, rabbit derived polyclonal antibody, ab138364), Akt (MCE, 1:2000, rabbit monoclonal antibody), p-Akt (MCE, 1:1000, rabbit monoclonal antibody, HY-P80276), mTOR (MCE, 1:1000, rabbit polyclonal antibody, HY-P80276), p-mTOR (MCE, 1:1000, rabbit monoclonal antibody, HY-P80837).

    Techniques: Western Blot

    Effects of D30 on PI3K-AKT pathway signaling and Gal-3 expression in vitro. (A, B) GO enrichment analysis (bubble map; A) and KEGG pathway analysis (histogram; B) performed as part of the pharmacological network analysis. (C) PI3K, p-AKT, AKT, p-mTOR, and mTOR expression in the hippocampus, as measured by immunoblot, after two fAβ injections. (D) Band densities are expressed as ratios of PI3K, p-AKT, AKT, p-mTOR, and mTOR to β-actin. (E) PI3K , AKT , mTOR , and Gal-3 mRNA expression levels in primary microglial cells stimulated with fAβ, as measured by qPCR. Values are expressed as the mean ± SEM ( n = 3–4). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s multiple comparisons post hoc test). AKT: Protein kinase B; D30: ( E )-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one; fAβ: fibrillar amyloid-β; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; mTOR: mammalian target of rapamycin; p-AKT: phosphorylated protein kinase B; p-mTOR: phosphorylated mammalian target of rapamycin; PI3K: phosphatidylinositol-3-hydroxykinase; qPCR: quantitative polymerase chain reaction.

    Journal: Neural Regeneration Research

    Article Title: The compound ( E )-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one alleviates neuroinflammation and cognitive impairment in a mouse model of Alzheimer’s disease

    doi: 10.4103/NRR.NRR-D-23-01890

    Figure Lengend Snippet: Effects of D30 on PI3K-AKT pathway signaling and Gal-3 expression in vitro. (A, B) GO enrichment analysis (bubble map; A) and KEGG pathway analysis (histogram; B) performed as part of the pharmacological network analysis. (C) PI3K, p-AKT, AKT, p-mTOR, and mTOR expression in the hippocampus, as measured by immunoblot, after two fAβ injections. (D) Band densities are expressed as ratios of PI3K, p-AKT, AKT, p-mTOR, and mTOR to β-actin. (E) PI3K , AKT , mTOR , and Gal-3 mRNA expression levels in primary microglial cells stimulated with fAβ, as measured by qPCR. Values are expressed as the mean ± SEM ( n = 3–4). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s multiple comparisons post hoc test). AKT: Protein kinase B; D30: ( E )-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one; fAβ: fibrillar amyloid-β; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; mTOR: mammalian target of rapamycin; p-AKT: phosphorylated protein kinase B; p-mTOR: phosphorylated mammalian target of rapamycin; PI3K: phosphatidylinositol-3-hydroxykinase; qPCR: quantitative polymerase chain reaction.

    Article Snippet: Next, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-glial fibrillary acidic protein (GFAP; rabbit, 1:8000, Proteintech, Wuhan, China, Cat# 16825-1-AP, RRID: AB_2109646), anti-Iba1 (goat, 1:1000, Abcam, Cat# ab5076, RRID: AB_2224402), anti-NeuN (rabbit, 1:1000, CST, Danvers, MA, USA, Cat# 24307, RRID: AB_2651140), anti-inducible nitric oxide synthase (iNOS; rabbit, 1:2000, Proteintech, Cat# 22226-1-AP, RRID: AB_2879038), anti-Aβ (rabbit, 1:1000, CST, Cat# 8243, RRID: AB_2797642), anti-phosphatidylinositol-3-hydroxykinase (PI3K; rabbit, 1:1000, CST, Cat# 4249, RRID: AB_2165248), anti-phosphorylated protein kinase B (p- AKT; rabbit, 1:2000, CST, Cat# 4060, RRID: AB_2315049), anti-AKT (rabbit, 1:2000, CST, Cat# 4691, RRID: AB_915783), anti-phosphorylated mammalian target of rapamycin (p-mTOR; rabbit, 1:1000, CST, Cat# 5536, RRID:AB_10691552), anti-mammalian target of rapamycin (mTOR; rabbit, 1:1000, CST, Cat# 2983, RRID: AB_2105622), anti-synaptophysin (rabbit, 1:5000, Abcam, Cat# ab32127, RRID: AB_2286949), anti-postsynaptic density protein 95 (PSD95; mouse, 1:3000, Abcam, Cat# ab13552, RRID: AB_300453), anti-Gal-3 (rabbit, 1:4000, Proteintech, Cat# 14979-1-AP, RRID: AB_2136768), anti-CD68 (rabbit, 1:2000, Proteintech, Cat# 28058-1-AP, RRID: AB_2881049), anti-C3 (rabbit, 1:3000, Abcam, Cat# ab97462, RRID: AB_10679468), anti-TNF-α (rabbit, 1:2000, Proteintech, Cat# 26405-1-AP, RRID: AB_2918102), anti-vesicular γ-aminobutyric acid transporter (VGAT; guinea pig, 1:2000, SYSY, Göttingen, Germany, Cat# 131-004, RRID: AB_887873), anti-β-actin (mouse, 1:6000, Proteintech, Cat# 66009-1-lg, RRID: AB_2782959), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; rabbit, 1:1000, CST, Cat# 5174, RRID: AB_10622025).

    Techniques: Expressing, In Vitro, Western Blot, Real-time Polymerase Chain Reaction